Neil Osheroff,Mary-Ann Bjornsti: DNA Topoisomerase Protocols : Volume II: Enzymology and Drugs


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Beginning with the Escherichia coli ? protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes have been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytotoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Part I: DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.
In his 15th parenting book, "Toilet Training Without Tantrums," nationally recognized expert John Rosemond lays to rest the notion that potty training must be the complicated, emotionally draining, stressful event it has become for children and parents. Your great-grandmother would be amazed to learn that toilet training has become one of Mom's greatest sources of anxiety and frustration during her child's early years. To Great-Grandma, it was no worse than teaching her child to use a spoon. Her Mother Was Crazy. Cat on a Hot Tin Roof first heated up Broadway in 1955 with its gothic American story of brothers vying for their dying father's inheritance amid a whirlwind of sexuality, untethered in the person of Maggie the Cat. The play also daringly showcased the burden of sexuality repressed in the agony of her husband, Brick Pollitt. In spite of the public controversy Cat stirred up, it was awarded the Pulitzer Prize and the Drama Critics Circle Award for that year. Williams, as he so often did with his plays, rewrote Cat on a Hot Tin Roof for many years the present version was originally produced at the American Shakespeare Festival in 1974 with all the changes that made Williams finally declare the text to be definitive, and was most recently produced on Broadway in the 2003-04 season. This definitive edition also includes Williams' essay "Person-to-Person," Williams' notes on the various endings, and a short chronology of the author's life. One of America's greatest living playwrights, as well DNA Topoisomerase Protocols : Volume II: Enzymology and Drugs free pdf as a friend and colleague of Williams, Edward Albee has written a concise introduction to the play from a playwright's perspective, examining the candor, sensuality, power, and impact of Cat on a Hot Tin Roof then and now. "
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Author: Neil Osheroff,Mary-Ann Bjornsti
Number of Pages: 334 pages
Published Date: 10 Nov 2010
Publisher: Humana Press Inc.
Publication Country: Totowa, NJ, United States
Language: English
ISBN: 9781617370571
Download Link: Click Here
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